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Directed Differentiation Of Multipotential Human Neural Progenitor Cells

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Title: Directed Differentiation Of Multipotential Human Neural Progenitor Cells
Description: Human neural progenitor cells were isolated under selective culture conditions from the developing human brain and directed through lineage differentiation to GFAP + (glial fibrillary acid protein) astrocytes. Following 3 weeks in astrocyte selective medium, cells were fixed and stained with antibodies to intermediate filament proteins that characterize the cells as either astrocytes (GFAP in orange) or neural progenitor cells (nestin in green). Nuclei are stained with DAPI in blue. Microscopic examination over the course of differentiation showed loss of progenitor cells (nestin down regulation) as cell population increasingly became astrocytes (GFAP up regulation). Photomicrograph was taken on Axiovert 200M Zeiss inverted microscope at 200 x magnification.
Categories: Research in NIH Labs and Clinics
Type: Color, Photo
Source: National Institute of Neurological Disorders and Stroke (NINDS)
Creator: Carol N. Ibe And Eugene O. Major
Date Created: Unknown
Date Added: 5/24/2012
Reuse Restrictions: None - This image is in the public domain and can be freely reused. Please credit the source and/or author listed above.

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